7ZWM
Pfs48/45 central and C-terminal domains bound to Fab fragments of monoclonal antibody 10D8 and 32F3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-29 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 108.583, 158.451, 186.163 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 93.790 - 3.690 |
R-factor | 0.2565 |
Rwork | 0.255 |
R-free | 0.27980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h5n |
RMSD bond length | 0.004 |
RMSD bond angle | 0.672 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | BUSTER (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 108.580 | 3.750 |
High resolution limit [Å] | 3.690 | 3.690 |
Rpim | 0.065 | 0.338 |
Number of reflections | 230227 | 11072 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | |
CC(1/2) | 0.971 | 0.438 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10 % (w/v) PEG monomethyl ether 5000, 0.1M 2-(N-morpholino) ethanesulfonic acid pH 6.5 and 12% (v/v) 1-propanol |