7ZVE
K403 acetylated glucose-6-phosphate dehydrogenase (G6PD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-28 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.363, 122.609, 161.310 |
| Unit cell angles | 77.23, 80.99, 77.40 |
Refinement procedure
| Resolution | 50.010 - 2.280 |
| R-factor | 0.21319 |
| Rwork | 0.211 |
| R-free | 0.26098 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qki |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.049 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.380 | 2.320 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rpim | 0.112 | |
| Number of reflections | 205547 | 10178 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 97.0 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.992 | 0.313 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 5% Tacsimate, 0.1M Hepes pH 7.0 10% PEG MME 5000 |
