7ZV9
Crystal structure of FLT3 in complex with a monomeric FLT3 Ligand variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 139.208, 139.215, 143.689 |
| Unit cell angles | 90.02, 90.03, 89.99 |
Refinement procedure
| Resolution | 47.900 - 4.507 |
| R-factor | 0.2784 |
| Rwork | 0.278 |
| R-free | 0.29130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qs7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.830 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.600 |
| High resolution limit [Å] | 4.300 | 4.300 |
| Number of reflections | 68007 | 1187 |
| <I/σ(I)> | 7.64 | 0.44 |
| Completeness [%] | 92.3 | 93 |
| Redundancy | 1.68 | 1.66 |
| CC(1/2) | 0.998 | 0.222 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 0.9 M ammonium sulfate 10% PEG 8000 MES buffer pH 8.3 |
