7ZUD

Crystal structure of HIV-1 capsid IP6-CPSF6 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-17
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9688
Spacegroup name	P 6
Unit cell lengths	90.741, 90.741, 56.588
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	78.580 - 2.930
R-factor	0.2777
Rwork	0.278
R-free	0.33600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h47
RMSD bond length	0.005
RMSD bond angle	0.740
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.580	3.230
High resolution limit [Å]	2.930	2.930
Number of reflections	5698	1717
<I/σ(I)>	7.6
Completeness [%]	98.4
Redundancy	1
CC(1/2)	0.984	0.425

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.3M Calcium chloride dihydrate, 0.3 M Magnesium chloride hexahydrate, 0.1 M Tris hydrochloride pH 8.5, 0.1 M Bicine, 20% PEG 4000