7ZTY

Structure of Vps39 N-terminal domain from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97937
Spacegroup name	P 21 21 2
Unit cell lengths	64.700, 102.750, 59.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.230 - 2.890
R-factor	0.2701
Rwork	0.269
R-free	0.29820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ALPHAFOLD
RMSD bond length	0.003
RMSD bond angle	0.551
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.820	3.070
High resolution limit [Å]	2.890	2.890
Rmeas	0.231	1.240
Number of reflections	17010	2619
<I/σ(I)>	7.7	1.8
Completeness [%]	99.1
Redundancy	6.72
CC(1/2)	0.993	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	20% PEG MME 2000, 0.1 M MES pH 7.25