7ZTW
Crystal Structure of Human Parechovirus 1 2A protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.548, 127.805, 54.282 |
| Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
| Resolution | 42.600 - 1.930 |
| R-factor | 0.1867 |
| Rwork | 0.184 |
| R-free | 0.23840 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.411 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.057 | 0.635 |
| Rpim | 0.053 | 0.579 |
| Number of reflections | 42961 | 2808 |
| <I/σ(I)> | 10.4 | 1.7 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl 0.2M Na acetate 10% glycerol 28% PEG1500 |
