7ZTR

Crystal structure of the carotenoid-binding protein domain from silkworm Bombyx mori (BmCBP) in the apoform, W232F mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-22
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	C 2 2 21
Unit cell lengths	62.033, 66.772, 120.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.250 - 1.750
R-factor	0.1836
Rwork	0.181
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ztq
RMSD bond length	0.015
RMSD bond angle	2.092
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.250	60.250	1.780
High resolution limit [Å]	1.750	9.090	1.750
Rmerge	0.086	0.047	0.848
Rmeas	0.090	0.052	0.952
Rpim	0.027	0.021	0.418
Total number of observations	289696	1212	5480
Number of reflections	25252	216	1143
<I/σ(I)>	20.8	39.5	1.8
Completeness [%]	98.5	98.8	84.8
Redundancy	11.5	5.6	4.8
CC(1/2)	0.979	0.994	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.5 M Sodium citrate tribasic dihydrate, 0.1 M BIS- TRIS propane, pH 7.0