7ZSZ

Crystal structure of the GH11 domain of a multidomain xylanase from the hindgut metagenome of Trinervitermes trinervoides

Replaces:  7AYL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 1
Unit cell lengths	38.490, 43.420, 69.900
Unit cell angles	100.87, 96.36, 116.00

Refinement procedure

Resolution	37.310 - 1.500
R-factor	0.1822
Rwork	0.180
R-free	0.21660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7AYL
RMSD bond length	0.006
RMSD bond angle	0.886
Data reduction software	XDS (1.12_2829)
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.310	1.413
High resolution limit [Å]	1.390	1.390
Rmerge	0.049	0.278
Number of reflections	68762	2652
<I/σ(I)>	13.6
Completeness [%]	87.5	67
Redundancy	1.6	1.6
CC(1/2)	0.999	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293.15	Na acetate, (NH4)2SO4