7ZS2
Diheme cytochrome c Kustd1711 from Kuenenia stuttgartiensis, M292H mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99988 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.650, 92.710, 55.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.510 - 1.120 |
| R-factor | 0.1261 |
| Rwork | 0.125 |
| R-free | 0.14260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WT Structure |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.523 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.150 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rpim | 0.042 | 0.892 |
| Number of reflections | 211675 | 4298 |
| <I/σ(I)> | 15.4 | 1.2 |
| Completeness [%] | 88.6 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M calcium acetate, 0.1 M imidazole/HCl pH 8.0 and 20% PEG 1K |
