7ZQU

A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - C72A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-06
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8726
Spacegroup name	C 1 2 1
Unit cell lengths	91.454, 28.210, 48.018
Unit cell angles	90.00, 119.40, 90.00

Refinement procedure

Resolution	41.840 - 1.750
R-factor	0.1886
Rwork	0.188
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kuk
RMSD bond length	0.006
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19rc6_4061)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.840	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.125
Rmeas	0.136
Rpim	0.053	0.912
Number of reflections	11015	593
<I/σ(I)>	7.5	0.8
Completeness [%]	99.8
Redundancy	6.4
CC(1/2)	0.996	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.1 M Tris, 0.2 M MgCl2, 20% PEG 8000