7ZQT

Helicobacter pylori adhesin BabA bound to neutralising human antibody.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.072
Spacegroup name	P 1 21 1
Unit cell lengths	99.700, 68.400, 176.600
Unit cell angles	90.00, 104.40, 90.00

Refinement procedure

Resolution	48.280 - 2.700
R-factor	0.228
Rwork	0.225
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f8r
RMSD bond length	0.009
RMSD bond angle	1.262
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	63386	1002
<I/σ(I)>	15.1
Completeness [%]	99.2
Redundancy	4.3
CC(1/2)	0.990	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	15% PEG6000, 0.1M NaAc pH 5.5, 0.2 M CaCl2