7ZQI

MHC class I from a wild bird in complex with a nonameric peptide P2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97940
Spacegroup name	P 21 21 21
Unit cell lengths	39.202, 48.780, 202.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.580 - 2.150
R-factor	0.18245
Rwork	0.180
R-free	0.22937
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gjx
RMSD bond length	0.013
RMSD bond angle	1.837
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.380	2.210
High resolution limit [Å]	2.150	2.150
Number of reflections	22044	1759
<I/σ(I)>	12.1	1.9
Completeness [%]	99.9	99.8
Redundancy	13	13.4
CC(1/2)	0.999	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100 mM Hepes/MOPS, pH 7.0, 20 mM MgCl2, 40 mM CaCl2, 25 % (v/v) MPD, 25 % (w/v) PEG 1000, 25 % (w/v) PEG 3350