7ZQ4

Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii (CrGAPA) complexed with NADP+ and the oxidated catalytic cysteine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	C 2 2 2
Unit cell lengths	142.396, 147.327, 74.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.180 - 1.700
R-factor	0.2175
Rwork	0.217
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rm4
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.430	46.430	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.083	0.050	1.197
Rmeas	0.086	0.052	1.243
Rpim	0.024	0.014	0.333
Total number of observations	1108041	8188	60509
Number of reflections	84958	663	4390
<I/σ(I)>	17.5	43.4	1.8
Completeness [%]	98.4	99.2	96.8
Redundancy	13	12.3	13.8
CC(1/2)	0.999	0.998	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	1.2-1.6 M (NH4)2SO4, 0.1 M Bicine pH 9.5