7ZPG

CRYSTAL STRUCTURE OF HUMAN MONOGLYCERIDE LIPASE WITH LIGAND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999993
Spacegroup name	C 2 2 21
Unit cell lengths	91.884, 127.430, 60.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.710 - 1.160
R-factor	0.1769
Rwork	0.176
R-free	0.18450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house pdb
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (3-FEB-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.710	1.260
High resolution limit [Å]	1.160	1.160
Rmerge	0.057	0.800
Number of reflections	122158	26486
<I/σ(I)>	2.7
Completeness [%]	99.8
Redundancy	6.39
CC(1/2)	0.999	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M MES 6.5 pH 10 %w/v PEG MME 5K 12 %v/v iso-propanol