7ZOU

Crystal structure of Synechocystis halorhodopsin (SyHR), Cl-pumping mode, ground state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 3 2 1
Unit cell lengths	61.980, 61.980, 109.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.950 - 1.580
R-factor	0.175
Rwork	0.173
R-free	0.20230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hyj
RMSD bond length	0.005
RMSD bond angle	0.837
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.680	1.610
High resolution limit [Å]	1.580	1.580
Rpim	0.059	0.993
Number of reflections	33368	1667
<I/σ(I)>	8
Completeness [%]	96.9
Redundancy	10.6
CC(1/2)	0.998	0.225

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4.6	293	1.6 M Ammonium Phosphate