7ZOF

Streptavidin Iron-Porphyrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	115.251, 89.439, 57.167
Unit cell angles	90.00, 97.54, 90.00

Refinement procedure

Resolution	44.760 - 1.740
R-factor	0.1714
Rwork	0.170
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ry2
RMSD bond length	0.010
RMSD bond angle	1.564
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.010	46.010	1.770
High resolution limit [Å]	1.740	9.040	1.740
Rmerge	0.050	0.017	1.400
Rmeas	0.059	0.020	1.648
Rpim	0.031	0.011	0.862
Number of reflections	58882	440	3209
<I/σ(I)>	20.1
Completeness [%]	100.0
Redundancy	6.9	6.3	7.1
CC(1/2)	1.000	1.000	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M MIB 7 pH (Buffer) 22.5 %v/v PEG 1500 (Precipitant)