7ZMX

Crystal structure of the Plant Homeodomain (PHD) of human ING3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	40.695, 40.695, 100.595
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.240 - 1.200
R-factor	0.1743
Rwork	0.172
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vnf
RMSD bond length	0.009
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.240	1.220
High resolution limit [Å]	1.200	1.200
Rmerge	0.085	0.613
Rmeas	0.094	0.723
Rpim	0.032	0.414
Number of reflections	47743	51
<I/σ(I)>	18.59	0.18
Completeness [%]	92.4	45.6
Redundancy	14.8	5.5
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	295	Protein solution: ING3 PHD at 20 mg/ml in Tris 20 mM pH = 6.5, 150 mM NaCl, 1 mM DTT. Reservoir solution: 0.1 M Tris pH = 8.4, 30% PEG 4000, 0.2 M calcium chloride Mixture of 1 ul of protein solution with 1 ul of reservoir solution.