7ZMV
Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G5-006
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9119 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.589, 89.965, 164.126 |
| Unit cell angles | 90.00, 110.06, 90.00 |
Refinement procedure
| Resolution | 77.687 - 2.002 |
| Rwork | 0.243 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lb5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.443 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.230 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 209231 | 9299 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 97.7 | 87.3 |
| Redundancy | 2.9 | |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M ammonium sulfate -- 25% PEG3350 -- 0.1M HEPES pH 7.5 |
