7ZMT
Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G5-006
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 82.355, 90.675, 217.853 |
| Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
| Resolution | 56.743 - 2.300 |
| Rwork | 0.264 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lb5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.522 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.940 | 2.160 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 89383 | 4291 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.3 | 95 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.900 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium formate -- 20% PEG3350 |
