7ZMM

Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G2-001

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	76.612, 183.969, 100.947
Unit cell angles	90.00, 108.12, 90.00

Refinement procedure

Resolution	95.938 - 2.500
Rwork	0.236
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lb5
RMSD bond length	0.006
RMSD bond angle	1.411
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	183.840	2.550
High resolution limit [Å]	2.500	2.500
Number of reflections	95510	4769
<I/σ(I)>	8.6
Completeness [%]	99.2
Redundancy	2.7
CC(1/2)	1.000	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M sodium chloride -- 25% PEG3350 -- 0.1M bis-tris pH 6.5