7ZMK

Structure of human MFAP4 in complex with the Fab fragment of the AS0326 monoclonal antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.041
Spacegroup name	C 1 2 1
Unit cell lengths	317.320, 126.600, 187.550
Unit cell angles	90.00, 98.86, 90.00

Refinement procedure

Resolution	48.850 - 3.400
R-factor	0.2401
Rwork	0.239
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m7h 4jpi
RMSD bond length	0.005
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.250	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.233
Number of reflections	100955	9948
<I/σ(I)>	5.3
Completeness [%]	99.8
Redundancy	4.4
CC(1/2)	0.993	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.14 M Ammonium phosphate dibasic, 14 % w/v Polyethylene glycol 3350