7ZLQ

Crystal structure of ADAR1-dsRBD3 dimer in complex with dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9999
Spacegroup name	P 61 2 2
Unit cell lengths	91.294, 91.294, 207.527
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.580 - 2.800
R-factor	0.2349
Rwork	0.231
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zj1
RMSD bond length	0.007
RMSD bond angle	0.878
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.580	44.580	2.990
High resolution limit [Å]	2.800	8.890	2.800
Rmerge	0.163	0.031	2.663
Rmeas	0.167	0.032	2.739
Rpim	0.038	0.010	0.633
Number of reflections	10044	502	503
<I/σ(I)>	16.6	61.8	1.2
Completeness [%]	94.9	100	88.6
Redundancy	19.2	16.1	18.3
CC(1/2)	1.000	1.000	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	50 mM sodium cacodylate, 5% (w/v) PEG 4000, 30 mM CaCl2, 230 mM KCl