7ZKZ
Crystal structure of cystinosin from Arabidopsis thaliana bound to two nanobodies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.281, 77.457, 46.303 |
| Unit cell angles | 90.00, 93.25, 90.00 |
Refinement procedure
| Resolution | 20.790 - 2.329 |
| R-factor | 0.2363 |
| Rwork | 0.235 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7zk1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 104.760 | 104.870 | 2.370 |
| High resolution limit [Å] | 2.329 | 6.320 | 2.330 |
| Rmeas | 0.185 | 0.064 | 2.387 |
| Rpim | 0.072 | 0.026 | 0.917 |
| Total number of observations | 10566 | 10356 | |
| Number of reflections | 31727 | 1659 | 1554 |
| <I/σ(I)> | 7.9 | 33.7 | 0.6 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 6.4 | 6.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.75 | 293 | 28% PEG 500DME, 100 mM MES-NaOH, pH 5.75, 150 mM K-formate |
