7ZKZ

Crystal structure of cystinosin from Arabidopsis thaliana bound to two nanobodies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	210.281, 77.457, 46.303
Unit cell angles	90.00, 93.25, 90.00

Refinement procedure

Resolution	20.790 - 2.329
R-factor	0.2363
Rwork	0.235
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zk1
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (20-OCT-2021))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	104.760	104.870	2.370
High resolution limit [Å]	2.329	6.320	2.330
Rmeas	0.185	0.064	2.387
Rpim	0.072	0.026	0.917
Total number of observations		10566	10356
Number of reflections	31727	1659	1554
<I/σ(I)>	7.9	33.7	0.6
Completeness [%]	100.0	99.9	100
Redundancy	6.4	6.4	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.75	293	28% PEG 500DME, 100 mM MES-NaOH, pH 5.75, 150 mM K-formate