7ZKR

Human GABARAP in complex with stapled peptide Pen3-ortho

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	I 2 3
Unit cell lengths	99.030, 99.030, 99.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.510 - 1.100
R-factor	0.1413
Rwork	0.140
R-free	0.16700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d32
RMSD bond length	0.005
RMSD bond angle	0.790
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.020	1.130
High resolution limit [Å]	1.100	1.100
Rmeas	0.132	1.694
Number of reflections	65281	4816
<I/σ(I)>	11.48	1.38
Completeness [%]	100.0
Redundancy	17.77
CC(1/2)	0.998	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	magnesium acetate, sodium cacodylate, PEG 8000