7ZJR

X-ray structure of the periplasmic region of PilJ from P. aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	80.059, 80.059, 202.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.440 - 2.503
R-factor	0.216598906847
Rwork	0.215
R-free	0.24524
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yfa
RMSD bond length	0.009
RMSD bond angle	0.904
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.440	2.460
High resolution limit [Å]	2.320	2.320
Rmeas	0.068	1.960
Number of reflections	54408	8751
<I/σ(I)>	21.36	1.07
Completeness [%]	99.9
Redundancy	7.8
CC(1/2)		0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% v/v Jeffamine M-600 pH 7.0, 0.1 M Tris pH 8.5