7ZJ1

Crystal structure of ADAR1-dsRBD3 dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 31 2 1
Unit cell lengths	44.235, 44.235, 131.803
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.150 - 1.650
R-factor	0.189
Rwork	0.187
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2mdr
RMSD bond length	0.010
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.150	19.150	1.709
High resolution limit [Å]	1.650	9.040	1.650
Rmerge	0.152	0.083	0.867
Rmeas	0.156	0.087	0.900
Rpim	0.035	0.022	0.238
Number of reflections	18792	138	1852
<I/σ(I)>	12.45	23
Completeness [%]	99.3	90.8	99.84
Redundancy	18.7	15.1	14.2
CC(1/2)	0.995	0.994	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	100 mM sodium citrate pH 4.0, 20% (w/v) PEG 6000, and 1.0 M LiCl