7ZIL
JC Polyomavirus VP1 in complex with 3'-Sialyllactose glycomacromolecules (aliphatic linker)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2022-02-05 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.098, 96.023, 128.058 |
| Unit cell angles | 90.00, 110.56, 90.00 |
Refinement procedure
| Resolution | 48.012 - 1.240 |
| Rwork | 0.138 |
| R-free | 0.15470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nxd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.659 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.012 | 1.310 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmeas | 0.070 | 1.040 |
| Number of reflections | 464154 | 61755 |
| <I/σ(I)> | 12.14 | |
| Completeness [%] | 95.9 | |
| Redundancy | 4.78 | |
| CC(1/2) | 0.990 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5, 0.2 M KSCN, 12% PEG 3350 |
