7ZHX

Leishmania donovani Glucose 6-Phosphate Dehydrogenase (N-terminal deletion variant)complexed with NADP(H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 2 2 2
Unit cell lengths	75.750, 229.660, 83.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.300 - 1.900
R-factor	0.1843
Rwork	0.182
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1292122100
RMSD bond length	0.009
RMSD bond angle	0.982
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	58616	32174
<I/σ(I)>	18.9
Completeness [%]	99.7
Redundancy	5.6
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	6 % PEG 3000, 200 mM AmCl2