7ZHW

Leishmania donovani Glucose 6-Phosphate Dehydrogenase complexed with NADP(H) and Glucose 6-Phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	C 1 2 1
Unit cell lengths	215.830, 66.770, 121.020
Unit cell angles	90.00, 120.71, 90.00

Refinement procedure

Resolution	49.300 - 3.300
R-factor	0.2386
Rwork	0.236
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1292122100
RMSD bond length	0.002
RMSD bond angle	0.615
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	3.400
High resolution limit [Å]	3.300	3.300
Number of reflections	22716	11462
<I/σ(I)>	9.7	1.3
Completeness [%]	99.1	98.6
Redundancy	5.1
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	12 % PEG 3000 and 200 mM ammonium sulfate