7ZGV
Serratia NucC bound to cA3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 114.265, 114.265, 96.069 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.850 - 1.480 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6q1h |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.133 |
| Data reduction software | XDS (BUILT=20190806) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.210 | 1.506 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.117 | 1.517 |
| Rpim | 0.027 | 0.354 |
| Number of reflections | 40117 | 1987 |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.9 | |
| CC(1/2) | 0.999 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 7.5 0.2 M calcium acetate 25% PEG MME 2000 |
