7ZGN

Plant/insect N-glycan active PNGase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	59.050, 100.550, 180.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.379 - 1.950
Rwork	0.189
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r4z
RMSD bond length	0.011
RMSD bond angle	1.768
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0326)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.090	1.990
High resolution limit [Å]	1.950	1.950
Number of reflections	79080	4454
<I/σ(I)>	12.6	1.7
Completeness [%]	99.9	99.6
Redundancy	7.2	7.2
CC(1/2)	0.999	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10 mM Cobalt chloride hexahydrate, 100 mM Tris pH 8.5 and 10% w/v Polyvinylpyrrolidone K15