7ZFM
Engineered Protein Targeting the Zika Viral Envelope Fusion Loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 81.618, 81.618, 113.282 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.220 - 1.711 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j2a |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Data reduction software | autoPROC (1.0.5) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 66.221 | 66.221 | 1.804 |
High resolution limit [Å] | 1.711 | 5.011 | 1.711 |
Rmerge | 0.045 | 0.024 | 1.095 |
Rmeas | 0.047 | 0.025 | 1.176 |
Rpim | 0.013 | 0.007 | 0.414 |
Total number of observations | 479564 | 23061 | 14162 |
Number of reflections | 37153 | 1857 | 1859 |
<I/σ(I)> | 28.45 | 85.39 | 1.47 |
Completeness [%] | 95.2 | 99.9 | 54.9 |
Redundancy | 12.91 | 12.42 | 7.62 |
CC(1/2) | 1.000 | 1.000 | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 1.0 M Succinic acid, 0.1 M Hepes pH 7.0 and 1% w/v PEG 2000 MME |