7ZFC

SARS-CoV-2 Beta RBD in complex with nanobody C1, Omi-18 and Omi-31 Fabs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	105.000, 105.000, 234.476
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	84.780 - 3.240
R-factor	0.2469
Rwork	0.244
R-free	0.29870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ps4
RMSD bond length	0.002
RMSD bond angle	0.508
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.000	3.290
High resolution limit [Å]	3.240	3.240
Rmerge	0.265
Rpim	0.073
Number of reflections	24674	1231
<I/σ(I)>	6.6	0.3
Completeness [%]	100.0
Redundancy	14.1
CC(1/2)	0.995	0.298

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.04 M Potassium phosphate monobasic, 16% w/v PEG 8000