7ZF8

SARS-CoV-2 Omicron BA.2 RBD in complex with COVOX-150 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-31
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	194.225, 84.933, 58.421
Unit cell angles	90.00, 101.02, 90.00

Refinement procedure

Resolution	57.340 - 2.950
R-factor	0.2221
Rwork	0.220
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bei
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.000	3.000
High resolution limit [Å]	2.950	2.950
Rmerge	0.490
Rpim	0.198
Number of reflections	19778	932
<I/σ(I)>	2.1	0.3
Completeness [%]	100.0	99.8
Redundancy	7
CC(1/2)	0.939	0.303

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium citrate tribasic dihydrate pH 5.5, 18% (w/v) PEG 3350