7Z7Q
Structure of the T207D single-point mutant of the fluorescent protein NeonCyan1 at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.063, 57.981, 71.446 |
| Unit cell angles | 68.57, 89.94, 89.58 |
Refinement procedure
| Resolution | 40.060 - 1.600 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ltr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.718 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.060 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.109 | 0.754 |
| Number of reflections | 85638 | 8266 |
| <I/σ(I)> | 8.8 | 2.4 |
| Completeness [%] | 86.7 | 83.6 |
| Redundancy | 1.7 | 2.6 |
| CC(1/2) | 0.993 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM HEPES pH 6.5 and 22% PEG8000 (w/v) |
