7Z7P
Structure of the fluorescent protein NeonCyan0.95 at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.150, 72.370, 117.360 |
| Unit cell angles | 90.19, 90.04, 90.07 |
Refinement procedure
| Resolution | 48.900 - 1.950 |
| R-factor | 0.2113 |
| Rwork | 0.209 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ltr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.711 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.078 | 0.868 |
| Number of reflections | 152394 | 15160 |
| <I/σ(I)> | 9.7 | 1.7 |
| Completeness [%] | 86.7 | 83.6 |
| Redundancy | 3.5 | 2.7 |
| CC(1/2) | 0.997 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate trihydrate (final pH 5.6), 24% PEG4000 (w/v), 0.2 M ammonium sulphate |
