7Z77
Crystal structure of compound 6 in complex with the bromodomain of human SMARCA2 and pVHL:ElonginC:ElonginB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.903, 63.707, 91.956 |
| Unit cell angles | 90.00, 101.32, 90.00 |
Refinement procedure
| Resolution | 30.060 - 1.970 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t35 4qy4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | STARANISO (2.2.19) |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.170 | 2.231 |
| High resolution limit [Å] | 1.970 | 1.974 |
| Number of reflections | 29493 | 1176 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 87.7 | |
| Redundancy | 5 | |
| CC(1/2) | 0.998 | 0.487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20 % PEG 3350, 0.1 M BIS_TRIS propane pH 7.5, 200 mM sodium formate |
