7Z74

PI3KC2a core in complex with PITCOIN2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	56.309, 135.151, 151.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.260 - 2.500
R-factor	0.2281
Rwork	0.226
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bi4
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.260	2.589
High resolution limit [Å]	2.500	2.500
Number of reflections	41035	4007
<I/σ(I)>	9.02
Completeness [%]	99.7	99.68
Redundancy	5.5
CC(1/2)	0.998	0.172

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	0.1MTris, 8-6% PEG3350, 0.2-0.1M MgSO4