7Z4V
Structure of Serine-Threonine kinase STK25 in complex with compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-21 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.200, 46.413, 57.954 |
| Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
| Resolution | 63.100 - 1.644 |
| R-factor | 0.247 |
| Rwork | 0.245 |
| R-free | 0.28340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kix |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.100 | 1.828 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.101 | 1.123 |
| Rmeas | 0.121 | |
| Rpim | 0.065 | |
| Number of reflections | 27829 | 1391 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 92.3 | 65.7 |
| Redundancy | 6.5 | |
| CC(1/2) | 0.995 | 0.678 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 22 % PEG 3350, 0.1 M Bis-tris propane pH 8.5, 0.2 M Potassium citrate, 10 % ethylene glycol |
