7Z3Q

Crystal structure of the human leptin:LepR-CRH2 encounter complex to 3.6 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-06
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 2
Unit cell lengths	156.197, 156.644, 95.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.570 - 3.617
R-factor	0.2774
Rwork	0.275
R-free	0.30530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ax8 3v6o
RMSD bond length	0.005
RMSD bond angle	0.740
Data reduction software	XDS (VERSION Feb 5, 2021 BUILT=20210205)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.7.0)
Refinement software	BUSTER (2.10.4 (3-FEB-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.320	3.940
High resolution limit [Å]	3.600	3.600
Rmeas	0.334	2.085
Number of reflections	13958	3298
<I/σ(I)>	6	1.2
Completeness [%]	100.0	100
Redundancy	9.8	10.1
CC(1/2)	0.994	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	Index screen HT #F1 0.2 M L-proline 0.1 M HEPES pH 7.5 10% PEG 3350 cryo: 25% PEG400