7Z1A

Nanobody H11 and F2 bound to RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 2
Unit cell lengths	157.010, 191.590, 56.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.160 - 2.590
R-factor	0.20991
Rwork	0.208
R-free	0.24067
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zbp
RMSD bond length	0.008
RMSD bond angle	1.613
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.160	2.660
High resolution limit [Å]	2.590	2.590
Rmerge	0.125
Number of reflections	54473	3936
<I/σ(I)>	14.6
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	0.999	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,000