7Z0F

CPAP:S-TUBULIN:IIH5 ALPHAREP COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	54.280, 86.710, 137.610
Unit cell angles	90.00, 96.61, 90.00

Refinement procedure

Resolution	45.790 - 2.396
R-factor	0.224
Rwork	0.222
R-free	0.27260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gwd
RMSD bond length	0.008
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.790	2.482
High resolution limit [Å]	2.396	2.396
Rmeas	0.083	1.556
Rpim	0.054
Number of reflections	49614	4790
<I/σ(I)>	13.06	1.05
Completeness [%]	99.2	95.8
Redundancy	4.47	4.51
CC(1/2)	0.998	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.2 M Na tartrate, 10% (W/V) polyethylene glycol 3350