7Z06
Structure of YwlG (Q2FF14) from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.741, 136.878, 79.190 |
| Unit cell angles | 90.00, 98.55, 90.00 |
Refinement procedure
| Resolution | 47.990 - 1.740 |
| Rwork | 0.250 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vd8 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.990 | 1.810 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.056 | |
| Number of reflections | 114645 | 10476 |
| <I/σ(I)> | 10.42 | |
| Completeness [%] | 97.8 | 88.8 |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.516 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 0.1 M BICINE 1.6 M Ammonium sulfate |
