7Z02

Z-SBTub2M photoswitch bound to tubulin-DARPin D1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	73.683, 91.615, 83.110
Unit cell angles	90.00, 97.39, 90.00

Refinement procedure

Resolution	45.810 - 2.360
R-factor	0.2084
Rwork	0.205
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nqt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.810	2.690
High resolution limit [Å]	2.360	2.360
Number of reflections	27889	1330
<I/σ(I)>	4.7	1.6
Completeness [%]	92.9
Redundancy	11.7
CC(1/2)	0.974	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium sulfate, 0.1M Bis/Tris-methane pH=5.5, 21% PEG 3000 (m/v)