7YZN

Structure of C-terminally truncated aIF5B from Pyrococcus abyssi complexed with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	72.260, 72.260, 205.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.750 - 1.700
R-factor	0.1702
Rwork	0.169
R-free	0.19640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Pa-aIF5B
RMSD bond length	0.010
RMSD bond angle	1.046
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.112	1.885
Number of reflections	61785	9577
<I/σ(I)>	20.2	1.88
Completeness [%]	99.5
Redundancy	26.3
CC(1/2)	0.999	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	8% Tacsimate; 20% PEG3350