7YZG
Crystal structure of the Xenopus FoxH1 bound to the TGTGGATT site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979280 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.304, 78.726, 103.253 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.600 - 2.820 |
R-factor | 0.2022 |
Rwork | 0.200 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7yz7 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.755 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.600 | 3.095 |
High resolution limit [Å] | 2.820 | 2.822 |
Rpim | 0.083 | |
Number of reflections | 5547 | 234 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 90.8 | 68 |
Redundancy | 14.7 | |
CC(1/2) | 0.997 | 0.484 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% PEG 8000, 0.2 M ammonium sulfate, 0.1 sodium cacodylate |