7YZ9
Structure of catalytic domain of Rv1625c bound to nanobody NB4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2020-02-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999879 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 94.793, 94.793, 119.655 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.070 - 1.970 |
| R-factor | 0.2076 |
| Rwork | 0.206 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p2f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.163 |
| Data reduction software | iMOSFLM |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 2.044 |
| High resolution limit [Å] | 1.970 | 1.973 |
| Rmerge | 0.090 | 1.587 |
| Number of reflections | 774280 | 1492 |
| <I/σ(I)> | 25.4 | 1.5 |
| Completeness [%] | 94.0 | 66.85 |
| Redundancy | 35.9 | 22.8 |
| CC(1/2) | 1.000 | 0.685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 0.1 M Na-acetate pH 5.5, 0.02 M CaCl2, 30% MPD |
