7YZ8
Triclinic crystal structure of YTHDF1 YTH domain (544AVV546 mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9429 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.225, 92.571, 113.940 |
| Unit cell angles | 72.87, 79.05, 67.87 |
Refinement procedure
| Resolution | 48.095 - 2.500 |
| R-factor | 0.1872 |
| Rwork | 0.184 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rci |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.050 | 49.050 | 2.540 |
| High resolution limit [Å] | 2.500 | 13.460 | 2.500 |
| Rmerge | 0.052 | 0.022 | 0.577 |
| Rmeas | 0.061 | 0.028 | 0.683 |
| Rpim | 0.033 | 0.016 | 0.360 |
| Total number of observations | 301916 | 1509 | 15646 |
| Number of reflections | 87250 | 515 | 4471 |
| <I/σ(I)> | 11.2 | 32.4 | 1.6 |
| Completeness [%] | 98.3 | 91.3 | 97.8 |
| Redundancy | 3.5 | 2.9 | 3.5 |
| CC(1/2) | 0.999 | 0.993 | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 6-12% PEG3350, 0.2 M KSCN |
