7YYK

Crystal structure of the O-fucosylated form of TSRs1-3 from the human thrombospondin 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9762
Spacegroup name	C 2 2 21
Unit cell lengths	66.153, 85.197, 62.121
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.25103
Rwork	0.249
R-free	0.31663
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lsl
RMSD bond length	0.013
RMSD bond angle	1.738
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.197	0.859
Number of reflections	5645	813
<I/σ(I)>	6.2	1.7
Completeness [%]	99.8
Redundancy	8.3
CC(1/2)	0.987	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 8000, zinc acetate, buffer MES