7YWE

Monocot chimeric jacalin JAC1 from Oryza sativa: dirigent domain (crystal form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-11
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	H 3
Unit cell lengths	158.900, 158.900, 47.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.870 - 2.150
R-factor	0.2196
Rwork	0.218
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ood
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MoRDa
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.870	2.210
High resolution limit [Å]	2.150	2.150
Number of reflections	24426	1815
<I/σ(I)>	7.83	0.68
Completeness [%]	99.7	99.9
Redundancy	4.22	4.36
CC(1/2)	0.998	0.353

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	ammonium dihydrogen phosphate, Tris-HCl, methylpentanediol, dithiothreitol